Table 1.
List of antibodies and dilution factor used for flow cytometry and western blot.
| Antibody | Company | Catlog Number | Dilution (Flow Cytometry) |
Dilution (Immunoblot) |
|---|---|---|---|---|
| Rabbit anti-DCLK1 | Sigma Aldrich | SAB4200186 | 1-500 | 1-500 |
| Mouse anti-CD133 | MACS | 130-092-395 | 1-50 | 1-50 |
| Mouose anti-ALDH1 | BD Biosciences | 611194 | 1-200 | 1-500 |
| Mouse anti-GAPDH | Santa Cruz | Sc51907 | - | 1-2000 |
| Rabbit anti-Jagged1 | Cell signalling | 2155S | - | 1-1000 |
| Rabbit anti-Notch1 | Cell signalling | 4380S | - | 1-1000 |
| Rabbit anti-Notch2 | Cell signalling | 5732S | - | 1-1000 |
| Rabbit anti-Notch3 | Cell signalling | 5276S | - | 1-1000 |
| Rabbit anti-Notch4 | Cell signalling | 2423S | - | 1-1000 |
| Rabbit anti-Presenilin 1 | Cell signalling | 5643S | - | 1-1000 |
| Rabbit anti-Nicastrin | Cell signalling | 5665S | - | 1-1000 |
| Rabbit anti-HES1 | Abcam | Ab71559 | - | 1-1000 |
| Rabbit anti-HEY1 | Abcam | Ab22614 | - | 1-1000 |