Table 3.

Mean peak areas (N = 3, each) of the legume marker peptides using different buffers at 90 °C and 2 h extraction time (most intense mass transitions as shown in Table 2; expressed as a percentage of the highest intensity (=100%; marked in bold); T = TRIS-HCl [1 M, pH 8.2], TE = TRIS-HCl [1 M, pH 8.2]/ethanol, TP = TRIS-HCl [1M, pH 8.2]/2-propanol, TA = TRIS-HCl [1 M, pH 8.2]/ACN) and using different extraction times. Extraction was performed with the buffer TA-60/40 at 90 °C for studies on extraction times.

	Extraction Buffer								Time (Min)
	T	TE	TP			TA
	100	50/50	50/50	60/40	70/30	50/50	60/40	70/30	30	60	90	120	150	180
Alfalfa 1	79	88	72	54	58	65	92	100	69	86	97	42	100	44
Alfalfa 2	14	35	41	52	45	67	100	75	93	89	66	92	91	100
Alfalfa 3	3	57	59	58	41	100	98	42	100	90	39	97	97	84
Broad bean 1	30	90	72	76	49	72	100	88	100	96	83	94	96	98
Broad bean 2	26	76	63	72	51	74	100	96	100	96	84	87	85	92
Broad bean 3	94	76	48	55	56	90	100	92	93	96	97	93	96	100
Chickpea 1	47	36	62	71	65	87	98	100	91	88	88	100	95	92
Chickpea 2	16	22	72	72	38	87	100	82	100	86	88	89	97	81
Chickpea 3	27	23	60	47	27	100	100	77	97	88	88	89	100	95
Lentil 1	12	20	76	74	39	87	100	79	79	88	90	94	100	96
Lentil 2	10	18	63	65	36	87	100	80	83	90	89	96	100	98
Lentil 3	7	14	36	67	36	83	100	78	92	89	92	100	100	98
Lupine blue 1	38	42	74	84	77	100	98	98	100	90	75	83	87	86
Lupine blue 2	19	17	45	54	40	92	100	89	100	100	70	83	81	80
Lupine blue 3	15	6	37	48	36	82	100	90	100	88	54	96	96	100
Lupine white 1	38	42	74	84	77	100	98	98	99	95	95	100	94	89
Lupine white 2	19	17	45	54	40	92	100	89	89	95	97	98	100	95
Lupine white 3	15	11	37	48	35	82	100	90	80	88	90	95	100	96
Pea 1	21	35	85	84	72	83	100	85	89	100	100	93	80	95
Pea 2	28	24	66	73	68	85	100	89	94	100	100	90	94	91
Pea 3	22	21	54	62	58	86	100	85	99	100	94	93	95	95
Peanut 1	69	43	71	70	61	98	100	87	100	86	81	71	61	56
Peanut 2	70	24	57	49	39	100	91	60	100	90	94	82	91	92
Peanut 3	73	59	81	71	72	100	82	59	100	83	85	72	79	80
Soy 1	29	29	67	67	58	96	100	93	100	80	77	77	73	68
Soy 2	6	16	52	43	27	100	98	68	100	95	87	94	88	77
Soy 3	28	33	77	60	53	95	100	86	100	92	89	92	86	75
Mean	31	35	61	64	50	88	98	83	95	91	85	89	91	87
Maxima (N)	0	0	0	0	0	7	19	2	13	4	2	3	7	3