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. 2021 Apr 23;12(5):633. doi: 10.3390/genes12050633

Figure 2.

Figure 2

miR-542-3p regulates the HK2-mediated high glycolytic phenotype in human glioma cells. (A) The ECAR levels in response to glucose, oligomycin, and 2DG (left) and quantification of ECAR levels (right) in microRNA hairpin inhibitor negative control treated (Control) and miR-542-3p knockdown (miR-542-3p KO) human glioma U87MG cells. Representative data are determined from three independent experiments. Data are mean ± SEM. ** p < 0.01; * p < 0.05 by two-tailed Student’s t-test. (B) Representative immunoblot analysis for HK2, PFKP, PKM2, and LDH-A protein levels (left) and quantification for HK2 and LDH-A protein levels (right) in microRNA hairpin inhibitor negative control treated (Control) and miR-542-3p knockdown (miR-542-3p KO) human glioma U87MG cells. In immunoblots, the levels of β-actin were used as loading control. Representative data are determined from three independent experiments. Data are mean ± SD. * p < 0.05 by the two-tailed Student’s t-test. (C) The ECAR levels in response to glucose, oligomycin, and 2DG (left) and quantification of ECAR levels (right) in microRNA mimic negative control treated (Control) and miR-542-3p over-expressed (miR-542-3p) human glioma U87MG cells. Representative data are determined from three independent experiments. Data are mean ± SEM. ** p < 0.01 by two-tailed Student’s t-test. (D) Representative immunoblot analysis for HK2, PFKP, PKM2, and LDH-A protein levels (left) and quantification for HK2 and LDH-A protein levels (right) in microRNA mimic negative control treated (Control) and miR-542-3p over-expressed (miR-542-3p) human glioma U87MG cells. In immunoblots, the levels of β-actin were used as loading control. Representative data are determined from three independent experiments. Data are mean ± SD. * p < 0.05 by the two-tailed Student’s t-test.