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. 2021 Apr 27;10(5):526. doi: 10.3390/pathogens10050526

Figure 6.

Figure 6

Comparison of HIV-1 infection and mock infection of Raji-CD4 cells in terms of EVs-borne miR-92, miR-155, and miR-223 distribution in the iodixanol velocity gradient. RT-PCR was used to measure microRNA in whole cells (A), or in a 100,000× g centrifugal pellet of the culture supernatant (B) of Raji-CD4 infected-cells. A pool from five plasma samples from HIV-1 negative individuals were spiked with virions or mock from Raji-CD4 infected-cells. Then EVs were precipitated by ExoQuickTM from plasmas. The pellet was re-suspended in PBS and laid on iodixanol velocity gradient. RNA was purified and miR-92 (C), miR-155 (D), and miR-223 (E) were amplified by RT-PCR and measured in each gradient fraction. Values are normalized as 38—Ct. Comparisons are based on two-way ANOVA with the Bonferroni post-test. (* p < 0.05, ** p < 0.01, *** p < 0.001). Values are mean ± SEM of at least three independent experiments.