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. 2021 Apr 27;10(5):526. doi: 10.3390/pathogens10050526

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Distribution of EVs-associated mitochondrial DNA in the iodixanol velocity gradient. Cell culture supernatant from uninfected cells was microfiltered then ultra-centrifuged at 100,000× g for 45 min. The EVs pellet was re-suspended in PBS. Total DNA was extracted from each fraction of the velocity gradient using the phenol/chloroform method and quantify by Biodrop (A). Mitochondrial DNA was quantified by qPCR using a standard curve and expressed as mean ± SEM of the technical triplicates of three independent cells preparation (B).