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. 2021 Apr 27;10(5):526. doi: 10.3390/pathogens10050526

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Iodixanol velocity gradient efficiently separates heterogeneous potential biomarkers from the plasma. The left panel represents non-exhaustive potential biomarkers present in the plasma. The middle panel illustrates that ExoQuick™ precipitates several biomarkers along with virions and calprotectin. The right panel illustrates that the velocity gradient might separate calprotectin, AChE-positive and Hsp-70-positive EVs, virions, and possibly new types of EVs enriched in miR-155 and mitochondrial DNA.