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. 2021 Apr 30;19(5):259. doi: 10.3390/md19050259

Figure 2.

Figure 2

SO decreased, while FO increased the mRNA expression of Pparγ and Srebp1c in 3T3-L1 mature adipocytes. The cells were differentiated to mature adipocytes in the presence or absence of various treatments for 8 days, and the mRNA expression of (A) peroxisome proliferator-activated receptor (Pparγ), and (B) sterol regulatory element-binding protein (Srebp1c) was measured as explained in the Methods section. The mRNA expression was normalized to RPLP0 as the reference gene, and expressed as Log2 fold change. Data were analyzed using one-way ANOVA and Tukey’s post-hoc test; p < 0.05 was considered significant. Superscripts (a, b, c) represent significant differences, n = 3. Untreated: Untreated cells; SE: Shrimp extract; SO: Shrimp oil; Astx-E: Esterified astaxanthin; FO: Fish oil; FOA = FO + Astx-E. Pparγ, untreated vs. SO (p = 0.0006); untreated vs. SE (p = 0.004); untreated vs. Astx-E (p = 0.0001); untreated vs. FO (p = 0.01); untreated vs. FOA (p = 0.01). Srebp1c, untreated vs. SO (p = 0.0004); untreated vs. SE (p < 0.0001); untreated vs. Astx-E (p = 0.001); untreated vs. FO (p = 0.01); FO vs. SO (p < 0.0001); FO vs. SE (p < 0.0001); FO vs. Astx-E (p < 0.0001).