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Iodine staining. Sporulating cells of 17 S. japonicus strains incubated on MEA medium at 30 °C for 2 days were stained by iodine vapor for 1 min. As reported previously, the type stain (FY16936) of S. japonicus var. japonicus was negatively stained, whereas the type stain (FY32181*) of S. japonicus var. versatilis was positively stained. Under this condition, TN33 was strongly positive; the others stains were negative (or weakly positive).
