Figure 5.

Shmooing assay using synthetic P-factor peptides. (A) Amino acid alignment of P-factor peptide from three Schizosaccharomyces species. Numerals to the right of each sequence indicate the length of the peptide product. Identical amino acids in the three peptides are highlighted by asterisks (*). (B) Shmoo formation in S. japonicus M cells lacking the sxa2⁺ gene (h⁻ sxa2Δ). Cells were treated with each of the synthetic P-factors at 5 µM and incubated in EMM2−N medium with gentle shaking for 24 hours. Arrow indicates a representative shmooing cell. Scale bar, 10 μm.