Figure 6.

Evolutionary relationship of S. japonicus strains. Phylogenetic tree of the 17 S. japonicus wild strains investigated in this study. The analysis combined multiple sequence alignments for the map2, map3, and mam2 genes. The evolutionary history was inferred by using neighbor-joining [35] and the Kimura 2-parameter method [36], and the number of base substitutions per site is shown. All ambiguous positions were removed for each sequence pair. In total, there were 2766 positions in the final data set. Evolutionary analyses were conducted in MEGA X [37,38]. Black and white stars indicate the type strain of S. japonicus var. japonicus and S. japonicus var. versatilis, respectively. S. japonicus var. versatilis strains were marked with an asterisk (*).