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The immunoblot analysis of the ALFPm3 derived from C. reinhardtii. Anti-His Tag: protein extracts incubated with mouse anti-His tag antibody for detecting His tag protein. Anti-α-tublin: protein extracts incubated with mouse anti-α-tublin antibody for detecting α-tublin. N: negative control, P: positive control, a protein with 6 × His tag expressed by E. coli with a concentration of 3.4 μg/mL. A1 to A3: transgenic Chlamydomonas.
