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. 2021 Apr 29;13(5):635. doi: 10.3390/pharmaceutics13050635

Figure 3.

Figure 3

CGFs induce the expression and release of VEGF and matrix metalloproteinases in endothelial cells. HUVECs were incubated with CGF-CM (10%) or culture medium (CTR) for 4 h and mRNA levels of VEGF. (A) MMP-9 and MMP-2 (C) were analyzed by qRT-PCR and expressed as fold over unstimulated control (CTR) (mean ± SD). HUVECs were incubated with CGF-CM (10%) or culture medium (CTR) for 4 h, and then culture media were removed and replaced with fresh media for 48 h. Media were collected, and VEGF (B), MMP-9, and MMP-2 (D) concentrations were analyzed by ELISA. Data are representative of three independent experiments, expressed as means ± SD. Each experiment consisted of four replicates for each condition. * p < 0.05 and ** p < 0.01 vs. control (CTR).