Table 1.
List of molecular probes (antibody, cytochemical reagent, and enzyme) used to detect infection thread cell wall and matrix components.
| Probe a | Component b | Epitope Recognized c | References |
|---|---|---|---|
| Antibody | |||
| JIM5 | low methyl-esterified HG | α-MeGalA(2)-(1→4)-α-GalA(3)- (1→4)-α-MeGalA |
[186,197,198,199,200,201,202,203,204] |
| JIM7 | high methyl-esterified HG | α-GalA-(1→4)-α- MeGalA(4)-(1→4)-α-GalA |
[197,202,203,204] |
| LM19 | low methyl-esterified HG | α-GalA-(1→4)(4) | [205] |
| LM20 | high methyl-esterified HG | α-MeGalA-(1→4)(4) | [205] |
| 2F4 | calcium cross-linked HG | dimer of α-MeGalA-(1→4)(9) and Ca2+(5) | [202] |
| LM5 | (1→4)-β-D-galactan (RG-I) | β-Gal-(1→4)(3) | [203,204] |
| anti-RG- II | monomeric and dimeric RG-II | unknown | [199,200,205,206] |
| anti- XyG | XyG | unknown | [197,207] |
| anti-callose | callose ((1→3)-β-D-glucan) | β-Glc-(1→3)(5) | [205] |
| MAC265 | 95kDa AGPE | unknown | [141,186,197,205,208,209,210,211] |
| MAC204 | 95kDa AGPE | unknown | [186,212,213] |
| MAC236 | 95kDa AGPE | unknown | [186,213] |
| JIM13 | AGP | unknown | [208] |
| anti-HRGP | hydroxyproline-rich glycoproteins (HPGPs) | unknown | [214] |
| anti-ENOD2 | early nodulin2/hydroxyproline-rich glycoproteins (ENOD2/HPGPs) | unknown | [215,216] |
| anti-VAMP721d/VAMP721e | vesicle-associated membrane proteins (VAMPs) | peptide QKLPSTNNKFTYNC | [205] |
| anti- EGL1 | endo-β-1,4-glucanase | peptide CYFPKRIHHRGSSLP | [217] |
| anti-LOX | lipoxygenase (LOX) | unknown | [218] |
| anti-SOD | superoxide dismutase (SOD) | unknown | [219] |
| anti-DAO | diamine oxidase (DAO) | unknown | [220] |
| Cytochemical reagent | |||
| chlor-zinc-iodide | cellulose | na | [15] |
| I2KI (I2, KI, H2SO4) | cellulose | na | [15] |
| cerium chloride (CeCl4) | H2O2 | na | [219,220,221,222,223] |
| ruthenium red | unesterified HG | na | [202,204] |
| aniline blue | callose ((1→3)-β-D-glucan) | na | [201,204] |
| neutral red | suberin | na | [201,204] |
| I2KI (I2, KI, H2SO4) | suberin | na | [204] |
| Protein | |||
| CBH-I | cellulose | na | [197] |
a JIM, John Innes Monoclonal, LM, Leeds Monoclonal, MAC, Monoclonal Antibody Centre (Babraham), RG-II. Rhamnogalacturonan-II, CBH-I. Cellobiohydrolase-I. b XyG. Xyloglucan, HG. Homogalacturonan, RG-I. Rhamnogalacturonan-I. c Gal, galactose, GalA, galacturonic acid, MeGalA, 6-O-methyl-galacturonate, na. not applicable.