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The best fit for concentration–current activation curve of WIN55,212-2 for CB-GIRK-RGS4 coupling. Oocytes were co-injected with CB, GIRK1/2, and RGS4 cRNAs. (A) CB1 activation and (B) CB2 activation were produced on the application of a range of different concentrations of WIN55,212-2 in the presence of HK. Each data point is the mean ± SD of three determinations from two or three batches of oocytes.
