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. 2021 Apr 28;11(5):279. doi: 10.3390/metabo11050279

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Conversion of EtAcAc to AcAc and stability of AcAc tracer. (A) Hydrolysis of EtAcAc to AcAc and EtOH (B) Real-time reaction monitoring by ¹H NMR. (C) Integrated ¹H NMR resonances of EtAcAc, AcAc, and EtOH over time. (D) The relationship between AcAc detected using ¹H NMR and an enzymatic assay. (E) Concentration of BHB and AcAc tracers during infusate preparation, double-tracer in vivo infusion in mice, and post-infusion. Dashed line denotes mixing of AcAc and BHB tracer solutions. (F) Measured AcAc tracer yield, data presented as mean and range.