Figure 7.

Modeling of the human E1o-E2o and of E1a-E2o sub-complex structures. (A) The E1o (α₂) and the E1a (α₂) homodimers are colored in yellow and in purple, respectively. The E2o core formed by tightly associated trimers is shown in violet. The E2o lipoyl domains are shown in dark blue. The N-terminal residues of E1o (residues 27–40) and E1a (residues 24–47) are shown in red. The E1o and E1a active centers located between the two subunits are shown in orange. (B) View of the E1o-E2o and E1a-E2o sub-complex structures at a different angle. Three regions of the E2o with α-helix secondary structure comprising residues 191–208 from the linker region (in blue), and residues 273–289 (in cyan) and 370–386 (in purple) both from the E2o core are indicated showing their different orientation versus E1o (left) and E1a (right) [94,104].