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. 2021 Apr 29;10(5):1047. doi: 10.3390/cells10051047

Figure 5.

Figure 5

Gray short-tailed opossum shares molecular characteristics of CatSper subunits conserved among eutherians. (A) mRNA expression pattern of CatSper subunits in developing testes of the gray short-tailed opossum. Relative expression levels of CatSper subunits are represented in a heatmap from the qRT-PCR analysis. CatSper expression in the testis is higher in adults (4–5 months and over 18 months; n = 3 each) compared to juvenile males (2 months old; n = 2). Expression levels in over 18-month-old males were set to 1-fold. (B,C) Conserved interaction of eutherian EFCAB9 and CatSperζ. EFCAB9 and CatSperζ from the same (B) or different (C) species were transiently expressed in 293T cells and co-immunoprecipitated. m, mouse; h, human. (D) Amino acid sequence alignment of human, mouse, and opossum EFCAB9. Marked are the annotated three EF-Hands in human and mouse EFCAB9. (E) Opossum EFCAB9 can form a complex with CatSperζ orthologs from other eutherian mammals. Transiently expressed opossum EFCAB9 and human (top) or mouse (bottom) CatSperζ in heterologous system are co-immunoprecipitated. See also Figure S4.