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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: FASEB J. 2020 Jul 1;34(8):10920–10930. doi: 10.1096/fj.201903093R

Figure 2. The effects of MβCD treatment on GlyR basal function.

Figure 2.

(a) Western blot of α1 GlyR and caveolin-1 in gradient fractions from HEK 293T cells transfected with α1 GlyRs after vehicle or MβCD pretreatment. (b) Current traces of IGly activated by various concentrations of glycine in GlyR α1-expressing HEK 293T cells pretreated with vehicle or MβCD (0.5%, 30 min). (c) Glycine concentration-response curves of HEK 293T cells transfected with α1 GlyR after vehicle (n=6) or MβCD (n=6) pretreatment. (d) The maximum current density of GlyR α1-expressing HEK 293T cells pretreated with vehicle (n=9) or MβCD (n=8). Data are represented as mean ± SEM. P value was determined based on unpaired t-tests with Welch’s correction.