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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: FASEB J. 2020 Jul 1;34(8):10920–10930. doi: 10.1096/fj.201903093R

Figure 4. Depletion of membrane cholesterol specifically affects cannabinoid-GlyR interaction.

Figure 4.

(a-e) Diagrams of chemical structures and the 10 μM AEA- (a), 1 μM CBD- (b), 100 μM propofol- (c), 100 μM isoflurane (d) and 100 mM ethanol- (e) induced potentiation of IGly in GlyR α1-expressing HEK 293T cells pretreated with vehicle or 0.5% MβCD for 30 min. (f) Current traces of IGABA activated by the EC2 concentration of GABA before and after 3 μM THC treatment in α1β2γ2 GABAAR receptor-expressing HEK 293T cells preincubated with vehicle or 0.5% MβCD. (g) Diagrams of THC structures and 3 μM THC-induced potentiation of IGABA in α1β2γ2 GABAAR-expressing HEK 293T cells pretreated with vehicle or 0.5% MβCD for 30 min. (h) Current traces of I5-HT activated by the maximally effective concentration (30 μM) of 5-HT before and during continuous incubation with 0.3 μM THC in 5-HT3A receptor-expressing HEK 293T cells pretreated with vehicle or 0.5% MβCD. (i) The percentage of THC-induced inhibition of I5-HT in HEK 293T cells expressing 5-HT3A receptors after vehicle or 0.5% MβCD treatment. Data are represented as mean ± SEM. **P<0.01, ***P<0.001 based on an unpaired t-test with Welch’s correction.