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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: FASEB J. 2020 Jul 1;34(8):10920–10930. doi: 10.1096/fj.201903093R

Figure 5. Membrane cholesterol and S296 are both necessary for THC-GlyR interaction.

Figure 5.

(a) Amino acid alignment of the third transmembrane helix in α1, α2, α3 and β subunits. (b) Current traces indicating THC (1 μM)-induced potentiation of IGly in HEK 293T cells expressing α1WT GlyRs and α1S296A GlyRs after 0.5% MβCD treatment. (c) Percentage of THC (1 μM)-induced potentiation of IGly in HEK 293T cells expressing α1WT, α3WT, α1S296A and α3S296A GlyRs after vehicle or 0.5% MβCD treatment. ***P<0.001 based on one-way ANOVA. (d) Percentage of THC (1 μM)-induced potentiation of IGly in HEK 293T cells expressing α2WT, α1βWT, α2A303S and α1βA320S GlyRs after vehicle or 0.5% MβCD treatment. All digits in the columns represent numbers of cells tested. Data are expressed as the mean ± SEM. ***P<0.001 based on one-way ANOVA.