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. Author manuscript; available in PMC: 2021 Dec 9.
Published in final edited form as: J Am Chem Soc. 2020 Nov 30;142(49):20513–20518. doi: 10.1021/jacs.0c09361

Figure 6:

Figure 6:

(A) Attachment of CB[7]-N3 to bicyclononyne (BCN)-functionalized Wang resin to produce Wang-CB[7]. (B) Wang-CB[7] was used to isolate payload, 13, from Jurkat lysate in 50% DMF/H2O (Step A), and was regenerated by complexation of 4 (Step B) followed by decomplexation (Step C). (C) Fluorescence of Wang-CB[7] throughout the recycling sequence (red) compared to fluorescent Wang-CB[7]•13 (green, samples not incubated with 4 in Step B) and non-fluorescent Wang-CB[7] (gray, samples not incubated with 13 or 4 in Steps A and B). Error bars represent standard deviation of three replicate samples. *P≤0.05, **P≤0.01, ***P≤0.001, ****P≤0.0001.