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Schematic of insulin granule isolation and purification techniques across the 4 ISG proteomes. Rat insulinoma cells in all ISG proteomic studies are lysed before ISG enrichment through various density gradients or immunoprecipitations. ISG are then purified prior to proteomics analysis by LC-MS/MS to obtain list of ISG proteins. Data collated from Brunner et al., 2007, Hickey et al., 2009, Schvartz et al., 2012 and Li et al., 2018.
