Figure 5.

Arsenic does not induce late lytic gene expression and inhibits virion production. (A) BX1-Akata cells were treated with 10 µM As₂O₃ or NaAsO₂ for 24 h, and BMRF1 RNA was measured by qRT-PCR. (B) BX1-Akata cells were treated with indicated doses of As₂O₃ or NaAsO₂ for 24 h, and immunoblot was performed to detect BMRF1 protein expression. (C) BX1-Akata cells were treated with indicated doses of As₂O₃ or NaAsO₂ for 24 h, and gp350 RNA was measured by qRT-PCR. (D) BX1-Akata cells were treated with either anti-IgG, 10 µM As₂O₃, or NaAsO₂ for 24 h, and immunofluorescence was performed to detect gp350 protein level. (E) BX1-Akata cells were treated with either anti-IgG, 10 µM As₂O₃, or NaAsO₂ for 4 days, and Raji cell infection assay was performed to determine infectious viral titers.