Figure 6.

BTdCPU induces EBV lytic gene expression. (A) BX1-Aakta cells were treated with 10 µM BTdCPU, and fluorescence microscopy was used for detecting GFP positive cells after 24 h of treatment. (B) BX1-Akata cells were treated with either As₂O₃, BTdCPU, or in combination (A+B) for 24 h, and qRT-PCR was performed. (C,D) BX1-Akata cells were transduced with lentiviral particles designed to knockdown PML, and cells were selected by puromycin. Immunoblot was performed to detect PML expression (C), and qRT-PCR was performed 24 h after As₂O₃ or BTdCPU treatment (D).