Table 2.
Chemical composition of monoterpene hydrocarbons, oxygenated monoterpenes, sesquiterpene hydrocarbons, oxygenated sesquiterpenes, total phenylpropanoids, classes, and the total identified extract from the cell suspensions of T. vulgaris and O. basilicum.
| No. | Compounds | RI (exp) | RI (lit) | T. Vulgaris | O. Basilicum |
|---|---|---|---|---|---|
| Relative Abundance % | |||||
| Monoterpene Hydrocarbons | |||||
| 1 | α-pinene | 937–934 * | 932 | 0.1 ± 0.02 | 0.1 ± 0.02 |
| 2 | camphene | 952–948 * | 946 | 0.1 ± 0.02 | 0.1± 0.02 |
| 3 | sabinene | 973–976 * | 973 | 0.1 ± 0.02 | 0.4 ± 0.06 |
| 4 | β-pinene | 977–978 * | 977 | 0.2 ± 0.02 | 1 ± 0.02 |
| 5 | β-myrcene | 991–992 * | 988 | 1.2 ± 0.2 | 0.1 ± 0.04 |
| 6 | α-phellandrene | 1005 | 1002 | 0.3 ± 0.02 | 0.1 ± 0.04 |
| 7 | car-4-ene | 1009 | 1004 | - | 1.2 ± 0.1 |
| 8 | α-terpinene | 1017–1018 * | 1014 | 1 ± 0.2 | 0.1 ± 0.1 |
| 9 | limonene | 1030 | 1224 | 0.5 ± 0.06 | 0.4 ± 0.1 |
| 10 | p-cymene | 1026–1023 * | 1023 | 17.3 ± 0.4 | - |
| 11 | (Z)-β-ocimeme | 1038–1040 * | 1032 | 0.5 ± 0.06 | 0.2 ± 0.02 |
| 12 | (E)-β-ocimeme | 1049–1050 * | 1044 | 0.1 ± 0.03 | 11.96 ± 0.2 |
| 13 | γ-terpinene | 1060–1061 * | 1067 | 9.1 ± 0.5 | 1.0 ± 0.2 |
| 14 | terpinolene | 1088 | 1086 | - | 0.7 ± 0.1 |
| Total Monoterpene Hydrocarbons Identified % | 30.5 ± 1.55 | 17.63± 1.02 | |||
| Oxygenated Monoterpenes | |||||
| 1 | 1,8-cineole (eucalyptol) | 1031–1026 * | 1031 | 1 ± 0.1 | 7.24 ± 0.4 |
| 2 | linalool oxide | 1094 | 1094 | 0.2 ± 0.04 | - |
| 3 | linalool (β-linalool) | 1099–1100 * | 1095 | 4.0 ± 0.8 | 1.2 ± 0.2 |
| 4 | β-terpineol | 1130 | 1130 | 0.2 ± 0.05 | 12.37 ±0.87 |
| 5 | camphor | 1145–1150 * | 1141 | 0.4 ± 0.03 | 1.4 ± 0.2 |
| 6 | cis-α-terpineol | 1143 | 1143 | 0.2 ± 0.04 | - |
| 7 | borneol (isoborneol) | 1167–1141 * | 1165 | 0.1 ± 0.02 | 0.2 ± 0.05 |
| 9 | terpinen-4-ol | 1177–1182 * | 1174 | 0.3 ± 0.02 | 2 ± 0.3 |
| 10 | dihydrocarvone | 1179 | 1179 | 0.1 ± 0.02 | - |
| 11 | α-terpineol | 1189 | 1186 | - | 0.1 ± 0.04 |
| 12 | estragole | 1199 | 1199 | - | 22.38 ± 0.7 |
| 13 | fenchyl acetate | 1214 | 1214 | - | 0.1 ± 0.02 |
| 14 | nerol | 1228 | 1227 | - | 1.6± 0.2 |
| 15 | thymol methyl ether | 1235–1161 * | 1235 | 1.7 ± 0.2 | - |
| 16 | neral | 1244 | 1244 | 0.05 ± 0.02 | 0.3 ± 0.1 |
| 17 | carvacrol methyl ether | 1248–1165 * | 1245 | 1.7 ± 0.1 | - |
| 18 | p-mentha-1,8-dien-7-ol | 1261 | 1261 | - | 0.1 ± 0.04 |
| 19 | thymol | 1264–1265 * | 1266 | 40.5± 0.86 | - |
| 20 | bornyl acetate | 1285 | 1284 | - | 0.5 ± 0.02 |
| 21 | carvacrol | 1299–1293 * | 1298 | 0.4 ± 0.04 | - |
| 22 | methyl geranate | 1321 | 1319 | - | 0.3 ± 0.03 |
| 23 | terpinyl acetate | 1333 | 1333 | 0.2 ± 0.03 | - |
| 24 | thymol acetate | 1349 | 1352 | 0.2 ± 0.04 | - |
| 25 | neryl acetate | 1364 | 1359 | - | 0.1 ± 0.04 |
| 26 | geraniol acetate | 1370 | 1368 | 1 ± 0.2 | - |
| 27 | methyl isoeugenol | 1492 | 1495 | 0.2 ± 0.02 | - |
| Total Oxygenated Monoterpenes Identified % | 52.45± 2.63 | 49.89± 2.88 | |||
| Sesquiterpene Hydrocarbons | |||||
| 1 | α-copaene | 1376 | 1374 | - | 0.4 ± 0.03 |
| 2 | α-cubebene | 1385–1386 * | 1387 | 0.2 ± 0.04 | 0.5 ± 0.02 |
| 3 | β-cubebene | 1389–1494 * | 1387 | 0.2 ± 0.03 | 0.7 ± 0.05 |
| 4 | β-elemene | 1391 | 1389 | - | 0.1 ± 0.03 |
| 5 | β-caryophyllene | 1424–1433 * | 1424 | 6.12 ± 0.1 | 1.1 ± 0.1 |
| 6 | trans-α-bergamotene | 1435 | 1432 | - | 0.2 ± 0.03 |
| 7 | α-guaiene | 1439 | 1437 | - | 4.8 ± 0.2 |
| 8 | β-bergamotene | 1441 | 1438 | - | 2.3 ± 0.2 |
| 9 | α-humulene | 1455 | 1452 | 0.5 ± 0.05 | 1.52 ± 0.04 |
| 10 | (E)-β-farnesene | 1457 | 1454 | - | 2.3 ± 0.08 |
| 11 | germacrene D | 1481 | 1484 | 0.4 ± 0.02 | 4.2 ± 0.2 |
| 12 | β-selinene | 1486 | 1489 | - | 0.2 ± 0.03 |
| 13 | β-bulnesene | 1505 | 1508 | - | 0.1 ± 0.02 |
| 14 | β-bisabolene | 1509 | 1512 | - | 0.3 ± 0.06 |
| 15 | γ-cadinene | 1513–1527 * | 1513 | 0.4 ± 0.03 | 0.7 ± 0.02 |
| 16 | δ-cadinene | 1525–1535 * | 1522 | 0.5 ± 0.05 | 0.4 ± 0.04 |
| 17 | α-cadinene (α-amorphene) | 1538–1487 * | 1537 | 0.4 ± 0.02 | 0.1 ± 0.02 |
| Total Sesquiterpene Hydrocarbons (SH) Identified % | 8.72 ± 0.34 | 19.92 ± 1.17 | |||
| Oxygenated Sesquiterpenes | |||||
| 1 | caryophyllene oxide | 1509–1512 * | 1507 | 0.4 ± 0.04 | - |
| 2 | cubenol | 1515 | 1514 | 0.4 ± 0.03 | - |
| 3 | τ-cadinol | 1640 | 1638 | 0.8 ± 0.05 | 0.1 ± 0.02 |
| 4 | α-eudesmol | 1653 | 1652 | - | 1.8 ± 0.1 |
| 5 | (2E,6Z)-farnesol | 1715 | 1712 | 0.2 ± 0.02 | - |
| Total Oxygenated Sesquiterpenes (OS) Identified (%) | 1.8 ± 0.14 | 1.9± 0.12 | |||
| Phenylpropanoids | |||||
| 1 | chavicol | 1256–1250 * | 1247 | 0.3 ± 0.05 | 0.1 ± 0.03 |
| 2 | eugenol | 1357 | 1356 | - | 3.7 ± 0.3 |
| 3 | methyl eugenol | 1406 | 1402 | - | 0.4 ± 0.02 |
| Total Phenylpropanoids (PP) Identified (%) | 0.3 ± 0.05 | 4.2 ± 0.35 | |||
| Non-Terpene Derivatives | |||||
| 1 | ethyl isovalerate | 853 | 856 | - | 0.3 ± 0.02 |
| 2 | 1-octen-3-ol | 981 | 981 | 1.32 ± 0.1 | - |
| 3 | 6-methyl-5-hepten-2-one | 985 | 988 | - | 0.4 ± 0.02 |
| 4 | 3-octanon | 989 | 988 | 0.1 ± 0.01 | - |
| 5 | 3-octanol | 996 | 996 | 0.2 ± 0.03 | - |
| Total Non-Terpene Derivatives (NT) Identified (%) | 1.62 ± 0.14 | 0.7 ± 0.04 | |||
| Total Identified (%) | 95.39 | 94.24 | |||
Value was obtained from 3 replicates (Mean ± Standard deviation (SD), n = 3); RI (exp): relative retention index determined on VF-5ms fused silica capillary column; * RI (exp) of T. vulgaris if the value is different from that obtained from the O. basilicum; RI (lit) relative retention index from MS libraries (Wiley); National Institute of Standards and Technology (NIST); Separation profiles of extracts after 40 days were explaining in Supplementary Figure S1.