Table 4.
In vitro specific activity of protease enzymes (OD/mg protein min) in R. ferrugineus larval instars ± SE treated with specific inhibitors.
| Inhibitors | Conc. (mM) | In Vitro (OD/mg Protein min) | |||||
|---|---|---|---|---|---|---|---|
| Total Proteases | Trypsin | Chymotrypsin | Elastase | Metallo-Protease | Cysteine Protease | ||
| Leupeptin A PMSF B TLCK C TPCK D EDTA E Iodoacetic acid F |
control | 25.83 ± 0.45 | 4.10 ± 0.11 | 7.35 ± 0.02 | 8.23 ± 0.02 | 1.15 | 0.59 ± 0.05 |
| 0.01 | 0.12 ± 0.05 | 3.90 ± 0.11 | 6.97 ± 0.03 | ND | ND | 0.28 ± 0.04 | |
| 0.05 | 0.08 ± 0.05 | 3.82 ± 0.1 | 6.40 ± 0.02 | ND | ND | 0.24 ± 0.05 | |
| 0.1 | 0.07 ± 0.05 | 3.57 ± 0.1 | 6.18 ± 0.02 | 6.46 ± 0.01 | 0.89 ± 0.11 | 0.19 ± 0.04 | |
| 1 | 0.01 ± 0.05 | 3.41 ± 0.09 | 5.28 ± 0.02 | 5.26 ± 0.03 | 0.80 ± 0.11 | 0.16 ± 0.05 | |
| 10 | ND | 3.08 ± 0.1 | 4.87 ± 0.02 | 4.18 ± 0.03 | 0.68 ± 0.11 | ND | |
| 50 | ND | 2.84 ± 0.09 | 3.64 ± 0.03 | 3.98 ± 0.0 | 0.59 ± 0.1 | ND | |
| 100 | ND | 2.12 ± 0.1 | 2.02 ± 0.02 | ND | 0.42 ± 0.1 | ND | |
(A) Inhibitor of general proteinase; (B) a general serine proteinase inhibitor (Elastase); (C) inhibitor of Trypsin; (D) TPCK as a chymotrypsin inhibitor; (E) an inhibitor of metalloprotease; (F) an inhibitor of cysteine protease; data are expressed as mean ± SE based on three replicates per tested concentration; ND: Not tested.