Figure 3. Wild-type (WT) Trp2/K^b-specific cells exhibit cell-intrinsic tolerance.

(A) We performed negative enrichment for CD8⁺ T cells from WT or Dct^-/- donors and transferred bulk CD8⁺ T cells into congenically distinct WT or Dct^-/- recipients. One day later, mice were immunized with TriVax (100 μg of Trp2 and B8R peptide). Donor and endogenous cells were collected from the spleens of recipient mice on day 7 following immunization and assessed for the percent (B, C) and number (D, E) of Trp2/K^b-binding cells. Data in B–E were compiled from three or more experiments. Squares indicate male animals. **p<0.05, ***p<0.001, ****p<0.0001 by one-way ANOVA with Sidak’s multiple comparisons test. Endog, endogenous; recip, recipients.

Figure 3—figure supplement 1. Response to infection with Listeria monocytogenes strain expressing Trp2 (LmTrp2) after adoptive transfer.

(A,B) CD8⁺ T cells from pre-immune wild-type (WT) (A) or Dct^-/- (B) donors were negatively enriched and bulk CD8⁺ T cells were transferred into congenically distinct WT or Dct^-/- recipients. One day later, mice were infected with LmTrp2. Donor and endogenous cells were collected from the blood of recipient mice on day 7 following infection and assessed for Trp2/K^b tetramer binding. Data represent individual experiments with two to five mice per group. ****p<0.0001 by one-way ANOVA with Sidak’s multiple comparisons test. Endog, endogenous; recip, recipients.