Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) | Dct-/- (Exon 2–6-/-) | Dr. A. Andy Hurwitz, NCI | ||
| Strain, strain background (Mus musculus) | C57BL/6NCrl (C57BL/6) | NCI Charles River | Strain code: 556 | |
| Strain, strain background (Mus musculus) |
B6.SJL-PtprcaPepcb/BoyCrCrl (CD45.1) | NCI Charles River | Strain code: 564 | |
| Strain, strain background (Mus musculus) | P14 | Dr. R. Ahmed, Emory University | ||
| Chemical compound, drug | Poly(I:C) | Invivogen | Cat. #: vac-pic | |
| Antibody | InVivoMAb anti-mouse CD40 | BioXCell | Cat. #: BE0016-2; RRID:AB_1107647 |
Clone FGK4.5 |
| Peptide, recombinant protein | Trp2 peptide | New England Peptide | Trp2180-188 H2N-SVYDFFVWL-OH | |
| Chemical compound, drug | 1-Fluoro-2,4-dinitrobenzene (DNFB) | Sigma Aldrich | Cat. #: D-1529 | |
| Antibody | Anti-mouse CD8a FITC | Tonbo Biosciences | Cat. #: 35-0081; RRID:AB_2621671 |
Clone 53-6.7 |
| Antibody | Anti-mouse CD8a vf450 | Tonbo Biosciences | Cat. #: 75-0081; RRID:AB_2621931 | Clone 53-6.7 |
| Antibody | Anti-mouse CD8a PerCP-Cy5.5 | Tonbo Biosciences | Cat. #: 65-0081; RRID:AB_2621882 | Clone 53-6.7 |
| Antibody | Anti-mouse CD8a APCef780 | eBioscience | Cat. #: 47-0081-80; RRID:AB_1272221 | Clone 53-6.7 |
| Antibody | Anti-mouse CD4 BV605 | BioLegend | Cat. #: 100548; RRID:AB_2563054 | Clone RM4-5 |
| Antibody | Anti-mouse CD4 PE-Cy7 | Tonbo Biosciences | Cat. #: 60-0042; RRID:AB_2621829 | Clone RM4-5 |
| Antibody | Anti-mouse CD44 BV786 | BD Biosciences | Cat. #: 563736; RRID:AB_2738395 | Clone IM7 |
| Antibody | Anti-mouse CD44 FITC | eBioscience | Cat. #: 11-0441-85; RRID:AB_465046 | Clone IM7 |
| Antibody | Anti-mouse CD44 rf710 | Tonbo Biosciences | Cat. #:80-0441; RRID:AB_2621985 | Clone IM7 |
| Antibody | Anti-mouse CD45.2 FITC | Tonbo Biosciences | Cat. #: 35-0454; RRID:AB_2621692 | Clone 104 |
| Antibody | Anti-mouse CD45.1 PE-Cy7 | Tonbo Biosciences | Cat. #: 60-0453; RRID:AB_2621850 | Clone A20 |
| Antibody | Anti-mouse CD90.1 ef450 | eBioscience | Cat. #: 48-0900-82; RRID:AB_1272254 | Clone HIS51 |
| Antibody | Anti-mouse CD90.2 PE-Cy7 | Tonbo Biosciences | Cat. #: 60-0903; RRID:AB_2621857 | Clone 30-H12 |
| Antibody | Anti-mouse MHC Class II (I-A/I-E) APC ef780 | Thermo Fisher Scientific | Cat. #: 47-5321-82; RRID:AB_1548783 | Clones M5/114.15.2 |
| Antibody | Anti-mouse MHC Class II (I-A/I-E) BV510 | BioLegend | Cat. #: 107635; RRID:AB_2561397 | Clones M5/114.15.2 |
| Antibody | Anti-mouse F4/80 BV510 | BioLegend | Cat. #: 123135; RRID:AB_2562622 | Clone BM8 |
| Antibody | Anti-mouse F4/80 APC ef780 | Thermo Fisher Scientific | Cat. #: 47-4801-82; RRID:AB_2735036 | Clone BM8 |
| Antibody | Anti-mouse CD122 BV421 | BD Biosciences | Cat. #: 562960; RRID:AB_2737918 | Clone TM-β1 |
| Antibody | Anti-human Granzyme B PE | Invitrogen/Thermo | Cat. #: GRB04; RRID:AB_2536538 | Clone GB11 |
| Antibody | Anti-mouse IFN-g PE-Cy7 | Tonbo Biosciences | Cat. #: 60-7311-U100; RRID:AB_2621871 | Clone XMG1.2 |
| Antibody |
Anti-mouse TNFa APC | eBioscience | Cat. #: 17-7321-81; RRID:AB_469507 | Clone MP6-XT22 |
| Antibody | Anti-mouse CD107a APC | BioLegend | Cat. #: 121614; RRID:AB_2234505 | Clone ID4B |
| Antibody | Anti-mouse IL-2 APC | BioLegend | Cat. #: 503810; RRID:AB_315304 | Clone JES6-5H4 |
| Antibody | Anti-mouse CD62L BV510 | BD Biosciences | Cat. #: 563117; RRID:AB_2738013 | Clone MEL-14 |
| Antibody | Anti-mouse CD62L PerCP-Cy5.5 | Tonbo Biosciences | Cat. #: 65-0621 | Clone MEL-14 |
| Antibody | Anti-mouse KLRG1 PE-Cy7 | eBioscience/Thermo | Cat. #: 25-5893-82; RRID:AB_1518768 | Clone 2F1 |
| Antibody | Anti-mouse CD127 BV786 | BD Biosciences | Cat. #: 563748; RRID:AB_2738403 | Clone SB/199 |
| Antibody | Anti-mouse CD69 FITC | Tonbo Biosciences | Cat. #: 35-0691; RRID:AB_2621698 | Clone H1.2F3 |
| Antibody | Anti-mouse CD25 PE-Cy7 | Thermo Fisher | Cat. #: 25-0251-82; RRID:AB_469608 | Clone PC61.5 |
| Antibody | Anti-mouse CD103 BV510 | BD Biosciences | Cat. #: 563087; RRID:AB_2721775 | Clone M290 |
| Antibody | Anti-mouse PD-1 PerCP-Cy5.5 | BioLegend | Cat. #: 135208; RRID:AB_2159184 | Clone 29F.1A12 |
| Antibody | Anti-mouse PD-1 FITC | BioLegend | Cat. #: 135214; RRID:AB_10680238 | Clone 29F.1A12 |
| Antibody | Anti-mouse Tim-3 BV421 | BioLegend | Cat. #: 119723; RRID:AB_2616908 | Clone RMT3-23 |
| Antibody | Anti-mouse CTLA-4 PE-Cy7 | BioLegend | Cat. #: 106314; RRID:AB_2564238 | Clone UC10-4B9 |
| Antibody | Anti-mouse CD5 APCef780 | Thermo Fisher scientific | Cat. #: 47-0051-82; RRID:AB_2573940 | Clone 53-7.3 |
| Antibody | Anti-mouse TCRβ APCef780 | Thermo Fisher Scientific | Cat. #: 47-5961-82; RRID:AB_1272173 | Clone H57-597 |
| Antibody | Anti-mouse LAG3 PE-Cy7 | BioLegend | Cat. #: 125226; RRID:AB_2715764 | Clone C9B7W |
| Antibody | Anti-mouse CD49a BUV395 | BD Biosciences | Cat. #: 740262; RRID:AB_2740005 | Clone Ha31/8 |
| Antibody | Anti-mouse CXCR3 PerCP-Cy5.5 | BioLegend | Cat. #: 126514; RRID:AB_1186015 | Clone CXCR3-173 |
| Antibody | InVivoMab anti-mouse IL-2 antibody | BioXCell | Cat. #: BE0043-1; RRID:AB_1107705 | Clone S4B6 |
| Chemical compound, drug | Recombinant Mouse IL-2 Protein, CF | R&D Systems | Cat. #: 402-ML-500/CF | |
| Chemical compound, drug | Streptavidin, R-Phycoerythrin Conjugate, premium grade | Invivogen | Cat. #: S21388 | |
| Chemical compound, drug | Streptavidin, Allophycocyanin Conjugate, premium grade | Invivogen | Cat. #: S32362 | |
| Chemical compound, drug | H-2K(b) SVYDFFVWL Monomer | NIH Tetramer Core | Biotinylated Monomer | |
| Chemical compound, drug | Ghost Dye Red 780 Viability Dye | Tonbo Biosciences | Cat. #: 13-0865-T100 | |
| Chemical compound, drug | LIVE/DEAD Fixable Aqua Dead Cell Stain Kit | Thermo Fisher | Cat. #: L34966 | |
| Chemical compound, drug | Annexin V FITC Apoptosis Detection Kit | BD Biosciences | Cat no. 556570; RRID:AB_2869085 |