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. 2021 Apr 30;10(5):1072. doi: 10.3390/cells10051072

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Secretome effects on inflamed chondrocytes. (A,B) Chondrocytes (CTRL) were treated with inflammatory cytokine without (IL-1β) and with secretome (IL-1β + SEC). VCAM1 and ICAM1 were detected by flow cytometry. Values on the y-axis show either the percentage of VCAM1-positive cells or the arbitrary amount (defined as Mean Fluorescence Intensity, being 100% chondrocytes positive in all conditions, CTRL set as 1) of ICAM1. N = 3, *** p ≤ 0.001, **** p ≤ 0.0001; ns, not significant. (C) Chondrocytes, as previously described, were counted and CTRL values set as 1. N = 3, *** p ≤ 0.001, **** p ≤ 0.0001; ns, not significant.