Figure 6.

The PLC-IP₃ pathway involvement. The Ca²⁺ response to 2% BH was significantly reduced in the presence of 2-APB, U73122, caffeine (30 min pre-incubation for each drug). The arrow shows BH addition after 60 s. Data are means ± s.e.m. of [Ca²⁺]_i traces recorded in different cells. Number of cells: BH honey: 40 cells from 3 exp; BH + 2-APB: 45 cells from 3 exp; BH + U73122: 45 cells from 3 exp; BH + caffeine: 45 cells from 3 exp. Insert. Mean ± s.e.m. of the Ca²⁺peak response. Number of cells as before. Asterisks on bars specify statistical differences with respect to CTRL determined by a Two-way ANOVA follow by Dunnett’s test (*** p < 0.001, **** p < 0.0001), or differences between BH treatments determined by a Two-Way ANOVA followed by Tukey’s test (§§§§ p < 0.0001).