Figure 4.

In vitro activity of MCT8-aAb. (A) Immunoprecipitation and Western blot analysis of recombinant human MCT8 by serum samples positive (P1–P3) or not (controls; C1–C4) for MCT8-aAb. The thick dark band in the lane far right presents the positive control (PC), i.e., an aliquot of recombinant MCT8 antigen as size marker and loading control. (B) Uptake of labeled T4 into MCT8-expressing MDCK-cells in culture in the presence of control (black squares, n = 3) or MCT8-aAb positive serum (green dots, P1 and P2). The T4 uptake dynamics were similar, but the extent of uptake was diminished by the presence of immunoglobulins isolated from the MCT8-aAb positive sera. Comparison by two-sided non-parametric U Mann–Whitney Test; * p < 0.05, *** p < 0.001.