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. 2021 May 1;10(5):911. doi: 10.3390/plants10050911

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Analysis of Chl fluorescence during photosynthesis under EL. (A) Chl fluorescence was monitored in cultures dark-adapted for 2 h. Cell suspensions were illuminated for 25 min and the non-photochemical quenching (NPQ) was determined at the end of light treatment (i.e., during steady-state photosynthesis). (B) PSII quantum yield recovery after EL treatment was quantified on WT and NFR strains by measuring F_v/F_m recovery in low light (20 μmol photons m⁻² s⁻¹, 24 °C, light yellow bar) upon 3 h of EL treatment (1800 μmol photons m⁻² s⁻¹, 24 °C, yellow bar). (Inset) kinetics of F_v/F_m were zeroed at the end of the EL treatment and normalized to the maximum F_v/F_m during low light recovery. Data are expressed as mean ± SD, n = 4.