Figure 4.

SPC microcrystals preserve nerve fibers and axons. A single unilateral subconjunctival injection of SPC microcrystals (200 μg in 5 μL) or vehicle (Sham) was prior to laser induced hypertension (n = 15–16). Optic nerves were evaluated for (A) number of nerve fibers and (B) percent reduction in nerve axons. Data shown as median ± IQR. * p < 0.05. Statistical analyses conducted by Wilcoxon rank sum test for two independent groups. (C) Retinal ganglion cell (RGC) loss was reduced with SPC microcrystals (n = 7) compared to untreated animals (n = 12), but not statistically significant (p = 0.08) due to variability and loss of tissues during the staining process. Data shown as mean ± SD. Statistical analysis conducted by unpaired t-test.