Effect of the combination of temperature shifts and different biomass concentrations on the course of dry matter accumulation (A), mean light availability (B), biomass starch content (C), volumetric starch concentration in the culture (D). A single C. reinhardtii culture was cultivated at 30 °C. After 4 days the culture was split into two, transferred to 39 °C and diluted to 0.1 mg mL−1 (dashed red lines) and 0.8 mg mL−1 (solid red lines), respectively. At day 7, the 0.1 mg mL−1 culture (dashed blue lines) and the 0.8 mg mL−1 culture (solid blue line) were transferred to 30 °C. Vertical lines at days 4 and 7 indicate those shifts in temperature (from 30 °C to 39 °C on day 4 and from 39 °C to 30 °C on day 7).