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. 2021 May 1;11(5):292. doi: 10.3390/metabo11050292

Figure 2.

Figure 2

Time-course of changes in gene and protein levels during the 60 days of differentiated adipocyte in conditioned medium. Aging adipocytes chronologically increased NF-κB p50 gene (A) and protein (B) expression while they decreased IκBα protein level (C). Gene and protein levels were measured by RT-PCR and Western blot, respectively, and normalized by GAPDH and EEF2 genes. Data were analyzed using one-way ANOVA, and significant differences were further analyzed using Tukey’s test; * p < 0.05, ** p < 0.01 compared to PID 10.