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Time course of changes in gene and protein levels during the 35 days of differentiated adipocytes in the unconditioned medium. mRNA of Ccl2 (A) and IL-6 (B) were measured by RT-PCR, and protein levels of NF-κB p50 (C) and IκBα (D) were measured by Western blot and normalized by EEF2 and GAPDH, respectively. Data were analyzed using one-way ANOVA, and significant differences were further analyzed using Tukey’s test; * p < 0.05, ** p < 0.01 compared to PID 10.
