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Pro-inflammatory gene expressions of EWATs and adipocytes of aged mice. EWATs were collected from YM and OM mice. The mRNA expressions of NF-κB p50 (A), Ccl2 (B), and TNF-α (C) were measured by RT-qPCR and normalized by GPDH and EEF2. Adipocytes were isolated from EWATs, and the mRNA expressions of NF-κB p50 (D), IL-6 (E), and TNF-α (F) were measured by RT-qPCR and normalized by GAPDH and EEF2. Data were analyzed using t-test; * p < 0.05 compared to YM.
