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. 2021 May 1;14(5):420. doi: 10.3390/ph14050420

Figure 1.

Figure 1

The detected variations in the mecA gene in the MRSA isolates (MR-13, -19, -4, -33, -26, -29, and -14). (a) Chromatograms showing nucleotide substitutions at different locations. (b) Multiple DNA sequence alignment. (c) Multiple protein sequence alignment. The sign “>” represents a substitution mutation. The left “E>K”, “E>R”, “G>E”, and the right “E>K” refer to E239K, E239R, G246E, and E447K, as shown by the dashed arrows. The left “G>A”, “A>G”, and “G>A”, and the right “G>A” refer to G715A, A716G, G737A, and G1339A. “Ref” represents the reference sequence (GenBank accession no. NC_002745). The MRSA isolates (MR-13, -14, and -33) show amino acid substitution(s) in PBP2a.