Fig. 7.

ZIKV infection induces upregulation of inflammatory and ER stress molecules and retinal cell death at P8. ZIKV was injected into neonatal mice at P0 and retinas were collected at P8. a, b Retinal RNA was extracted and analyzed by qPCR for the expression of inflammatory genes and ER stress genes. n = 5–6 per group. c Immunostaining with antibodies against cleaved caspase3 (upper panel) and pRIP3 (lower panel) in retinal sections of control and ZIKV-infected mice. Blue: DAPI staining for nuclei. Arrows indicate apoptotic or necroptotic cells. Scale bar = 50 μm. n = 3–4 per group. Data are presented as mean ± S.E.M. *p < 0.05 **p < 0.01, ***p < 0.001 compared to control mice