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. 2021 May 25;9:97. doi: 10.1186/s40478-021-01195-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 9 — The effects of live attenuated ZIKV vaccine candidate on retina. Various dose of a live-attenuated ZIKV vaccine candidate (Vac-20 or 200 or 2000 PFU) was subcutaneously injected into neonatal mice at P0 and retinas were collected at P21. a Representative images of PI-stained retinal sections. Stars indicate retinal samples with decreased thickness. b–f Representative images of retinal flatmounts labeled with anti-CD31 for vessels, anti-Tuj1 for RGCs and their axons, anti-GFAP for astrocytes, anti-Iba1 for microglia, and anti-CD45 for leukocytes. Scale bar = 50 μm except b where scale bar = 500 μm. n = 5 for Vac-20; n = 8 for Vac-200 and Vac-2000