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. 2021 May 1;14(5):421. doi: 10.3390/ph14050421

Figure 2.

Figure 2

ICG-001 inhibits β-catenin dependent transcription in KHOS, MG63 and 143B osteosarcoma cell lines. (a) Relative Luciferase Firefly/Renilla activity ratio in cells co-transfected with TOPFLASH and pRL-TK constructs and treated or not (CT) with vehicle (Vh) or ICG-001 (1 to 10 µM). Wnt3a 25 ng/mL was added 1 h after ICG-001 treatment for a period of 24 h. Bars represent means ± SD of 5 independent experiments. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 vs. vehicle. (b) Relative and normalized AXIN2 or BIRC5 gene expression determined by RT-qPCR in cells treated with vehicle (Vh) or 10 µM of ICG-001 during 24 h (right panel). Bars represent means ± SD (n = 3, performed in duplicate). * p < 0.05 vs. vehicle. (c) SURVIVIN and TUBULIN protein level evaluated by western blot in cells treated with vehicle (Vh) or ICG-001 (1–10 µM) during 48 h (upper panel with one representative experiment of three independent experiments). Relative SURVIVIN protein quantification, normalized with TUBULIN protein expression (lower panel). Bars indicate means ± SD of three independent experiments. ** p < 0.01 vs. vehicle.