Table 1.

Raw read data summary for unfiltered, Albacore “passed” and filtered Oxford Nanopore long-read sequencing datasets for Thalassiosira pseudonana and Phaeodactylum tricornutum. The unfiltered data include all sequence reads, including passed (q-score>7) and failed (q-score<7) reads as determined by Albacore. The Albacore “pass” data include all reads with a quality-score >7. The filtered datasets for T. pseudonana and P. tricornutum included reads with read length ≥30 kb and ≥20 kb, respectively

		Unfiltered Data	Albacore “pass” Data	Filtered Data
Phaeodactylum tricornutum	Total bases (Gbp)	8.2	7.5	2.7
	No. of reads	986,604	820,187	84,445
	Mean read length (bp)	8,311.1	9,144.7	31,973.9
	Mean read quality	8.5	9.2	9.6
	Read length N50 (bp)	18,756	19,261	32,648
	Estimated genome coverage	~300x	~273x	~100x
	Percentage of reads mapped to JGI reference	76.8%	87.8%	92.6%
	Average percent identity of reads to JGI reference	73.7%
Thalassiosira pseudonana	Total bases (Gbp)	7.5	7.0	1.8
	No. of reads	701,596	580,845	46,708
	Mean read length (bp)	10,611.8	12,029.7	37,942.3
	Mean read quality	9.9	10.8	10.9
	Read length N50 (bp)	20,088	20,514	37,303
	Estimated genome coverage	~230x	~215x	~50x
	Percentage of reads mapped to JGI reference	76.6%	89.1%	93.5%
	Average percent identity of reads to JGI reference	71.5%