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Optimization of an indirect ELISA utilizing SARS-CoV-2 NP recombinant protein. The assay was conducted as described in Section 2.3. (A) A representative image of results obtained at a range of SARS-CoV-2 NP coating concentration (6.25 to 200 ng) and a positive sample dilution (1:100 to 1:3200). Negative control and blank were also included. (B) Representative OD450 readings. (C,D) The effect of antigen (Ag) coating concentration and sample dilution on signal readings.
