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. 2021 May 2;11(5):825. doi: 10.3390/diagnostics11050825

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Validation of the developed COVID-19 NP IgG ELISA. (A) The cut-off value of the assay. Negative samples (red) and positive control (green) based on micro-neutralization assay in addition to blank (black) were utilized. The actual OD₄₅₀ values for each sample are shown. Dashed lines represent the cut-off value 0.17, which was calculated as mean + (3 × standard deviation). (B) Positive samples (green) and negative control (green) based on micro-neutralization assay in addition to blank (black) were utilized. OD₄₅₀ values for all positive samples were above the cut-off value. (C) correlation between ELISA results and MN titer. One-way ANOVA was applied. * indicates p value < 0.05.