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. 2021 May 2;11(5):825. doi: 10.3390/diagnostics11050825

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Evaluation of cross-reactivity. (A) Human sera containing IgG antibodies to SARS-CoV-2 (green), MERS-CoV (blue), or HCoV-HKU1 (purple) were subjected to the developed COVID-19 NP IgG ELISA. (B) Samples obtained for vaccinated people (blue) or recovered patients (green) were tested for the presence of IgG antibodies directed to SARS-CoV-2 S protein (left panel) and NP protein (right panel). Negative control (red) and blank (black) were included in all experiments. Dashed lines represent the assay cut-off value.