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. 2021 May 24;14:278. doi: 10.1186/s13071-021-04764-9

Table 1.

Primer sets of the loop-mediated isothermal amplification for detection of Malaria Pan, Plasmodium vivax and P. ovale

Species Primer Sequence (5′–3′)
Malaria Pan LAMP assay F3 TGTCAACTACCATGTTACGAC
B3 AACGGTCCTAAGGTAGCAA
FIP (F1c-F2) TACGGCCCGACGGTAAGATCGTAACCATGCCAACAC
BIP (B1c-B2) AGGAGTCTCACACTAGCGACAAAATTCCTTGTCGGGTAATCTC
LPF CTGAGCACCTTAACTTCCCTAA
LPB TACACCGTTCATGCAGGAC
P. vivax F3 CTAATTAGCGGTAAGTACGACA
B3 AGCCTAGTTCATCTAAGGACA
FIP (F1c-F2) ACCAAACGCATCAGCTATTCGTATGTCGGATTGGATCTGGA
BIP (B1c-B2) TTACTTGGCTTATCGTACCGTTCAGACCTGTTGTTGCCTT
LPF CACCGACACGAAGTATAATTGC
LPB GCTTCTTAGAGGAACGATGTGT
P. ovale F3 CGAGTTTCTGACCTATCAGC
B3 GCTGGCACCAGACTTG
FIP (F1c-F2) GATGTGGTAGCTATTTCTCAGGCTCCCTAACATGGCTATGACGG
BIP (B1c-B2) GCAGCAGGCGCGTAAATTACAACCATGAAATGGCCTTGT
LPF TCTCCGGAATCGAACTCTAATTC
LPB TCTAAAGAAGAGAGGTAGTGACAAG

LAMP loop-mediated isothermal amplification