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. 2021 May 15;2021:6622342. doi: 10.1155/2021/6622342

Figure 2.

Figure 2

miR-126-5p increases NSCLC cell apoptosis and suppresses the cell proliferation through inhibiting the PTEN/PI3K/Akt pathway: (a) qRT-PCR-based detection of transfection efficiency of miR-126-5p mimics; (b) MTT assay-based detection of A549/DDP cell proliferation in blank, NC, and mimics groups; (c) MTT assay-based detection of H1650/DDP cell proliferation in the three groups; (d) colony formation assay-based detection of proliferation of the indicated cells in the three groups; (e) flow cytometry-based detection of apoptosis of the indicated cells in the three groups; (f) (left one and two) western blotting-based detection of PTEN expression and key components within the PI3K/Akt pathway in the indicated cells; (right one and two) quantification of the gray values of protein bands in the blots. ∗P < 0.05, ∗∗P < 0.01, compared with the NC group.