Fig. 2.

VWF multimer analysis and plasma levels of VWF antigen and ADAMTS13 activity

VWF multimer analysis using SDS-1.0% agarose electrophoresis revealed a reduction in the level of high-molecular weight VWF multimers (HMW-VWFMs) during ECMO support. VWF multimers were classified as HMW-VWFMs if they corresponded to bands > 10 in the VWF multimer analysis. Plasma levels of VWF antigen were extremely high, and ADAMTS13 activity was moderately reduced during the hospitalization. Values of 100% were defined as the amounts of VWF antigen, and ADAMTS13 activity in pooled plasma from normal healthy volunteers.

NP: normal human plasma, ECMO: veno-venous extracorporeal membrane oxygenation