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. 2021 May 25;7:38. doi: 10.1038/s41421-021-00275-0

Fig. 2. Knockdown of PKR and G3BP1 facilitates viral replication.

Fig. 2

a, b Deficiency of PKR or G3BP1 promotes the transcription of SARS-CoV-2 genes. HeLa-ACE2 cells were transfected with siRNAs specific for PKR (siPKR) or G3BP1 (siG3BP1) or control siRNA (siNC) for 48 h. The cells were then infected with SARS-CoV-2 (MOI = 0.01) for 24 h before qPCR analysis with the indicated primers. c, d Knockdown of PKR or G3BP1 promotes SARS-CoV-2 replication. HeLa-ACE2 cells were transfected with siRNAs specific for PKR (siPKR) or G3BP1 (siG3BP1) or control siRNA (siNC) for 48 h. The cells were then infected with SARS-CoV-2 (MOI = 0.01) for 24 h. Virus yield in the infected cell supernatants was quantified by qPCR analysis with primers based on RBD of SARS-CoV-2 S gene (c), and NP expression in infected cells was analyzed by immunoblot with the indicated antibodies (d). Graphs show means ± SD, n = 3. *P < 0.05, **P < 0.01 (Student’s t-test).