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. 2021 May 3;9(5):102. doi: 10.3390/toxics9050102

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Assessment of hatchability and heart rate of zebrafish embryos. (A) Percentage of embryos hatching of zebrafish treated with ZO essential oil. (B) Correlation between the dose of ZO essential oil and zebrafish larvae heart rate (bpm) at 72 hpf. Data are presented as mean ± SD of three independent experiments (n = 60 embryos/group). Data were analyzed using one-way ANOVA and Tukey’s multiple comparison test. Different lowercase letters indicate significant differences between groups (p < 0.05).